منابع مشابه
Basic principles of real-time quantitative PCR.
Real-time quantitative PCR allows the sensitive, specific and reproducible quantitation of nucleic acids. Since its introduction, real-time quantitative PCR has revolutionized the field of molecular diagnostics and the technique is being used in a rapidly expanding number of applications. This exciting technology has enabled the shift of molecular diagnostics toward a high-throughput, automated...
متن کاملAdvances in real-time PCR: application to clinical laboratory diagnostics.
The polymerase chain reaction (PCR) has become one of the most important tools in molecular diagnostics, providing exquisite sensitivity and specificity for detection of nucleic acid targets. Real-time monitoring of PCR has simplified and accelerated PCR laboratory procedures and has increased information obtained from specimens including routine quantification and differentiation of amplificat...
متن کاملApplication of Real-time Polymerase Chain Reaction (RT-PCR)
The real-time polymerase chain reaction (RT-PCR), also called quantitative real-time polymerase chain reaction (QRT-PCR) or kinetic polymerase chain reaction (kPCR), is a technique used to simultaneously quantify and amplify a DNA molecule. It is used to determine whether a specific DNA sequence is present in the sample; and if it is present, the number of copies in the sample. It is the real-t...
متن کاملReal-Time PCR Platforms
Real-time PCR continues to have a major impact across many disciplines of the biological sciences and this has been a driver to develop and improve existing instruments. From the first two commercial platforms introduced in the mid 1990s, there is now a choice in excess of a dozen instruments, which continues to increase. Advances include faster thermocycling times, higher throughput, flexibili...
متن کاملPerforming Real-Time PCR
Optimisation of the reagents used to perform PCR is critical for reliable and reproducible results. As with any PCR initial time spent on optimisation of a real-time assay will be benefi cial in the long run. Specifi city, sensitivity, effi ciency and reproducibility are the important criteria to consider when optimising an assay and these can be altered by changes in the primer concentration, ...
متن کاملReal-time PCR Statistics
Real-time PCR is a powerful tool to compare relative transcriptional abundance, and has been broadly applied in biomedical sciences. However, methods for rigorous statistical analyses have lagged behind applications. Confidence interval and statistical significance considerations are not explicit in many of the current data analysis approaches. Based on the standard curve method and other usefu...
متن کاملمنابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
hepatitis monthlyجلد ۲۰۱۶، شماره ۱۱، صفحات ۸۳-۸۷
میزبانی شده توسط پلتفرم ابری doprax.com
copyright © 2015-2023